Lichtgedanken 04

S C HW E R P U N K T 33 04 | LICHT GEDANKEN Head of a fly This image, which was taken using a light-sheet microscope, offers an insight into the throat area of a fruit fly (Drosophila melanogaster). The sample is hit from the side with a »light wedge« which is around one thousandth of a millimetre in size and can be moved around. The head is half a millimetre in size. Taken by Ulrich Leischner/Department of Mi­ croscopy, Leibniz Institute of Photonic Technology C Nanoparticles The cryo-electron microscopy image shows snail-like coils of micelles of block copolymers in an aqueous solution, which have been formed by self-organi­ sation. The diameter of the micelles is around 200 nanometres (0.2 micrometres or 0.0002 millimetres). Taken by Dr Stephanie Höppener/Jena Centre for Soft Matter Cytoskeleton The fluorescent microscopic image shows so-called microtubules. They are protein filaments from the cytoskeleton of cells, which contribute to their stabil­ ity. The image compares two microscopic methods. The right half of the image shows an image of the cell taken with a conventional wide-field fluorescent microscope; whilst the left half of the image shows the result of high-resolution localisation microscopy dSTORM (direct Stochastic Optical Reconstruction Microscopy). The width of the section shown is approx. 40 micrometres (0.04 millimetres). Taken by Patrick Then/Department of Microscopy, Leibniz Institute of Photonic Technology High-resolution immune cells High-resolution image of fluorescently marked actin filaments (structural proteins, blue) in white blood cells (T-lymphocytes) in suspension. The image shows a 3D reconstruction of scanned image stacks. Top left: images with a confocal laser scanning microscope; bottom right: images from a high- resolution STED microscope (Stimulated Emission Depletion). The lymphocyte has a diameter of around 10 micrometres (0.01 millimetres). Taken by Marco Fritzsche/Mathias Clausen, AG Christian Eggeling/ Institute of Applied Optics Endothelial cell The fluorescent microscopic image shows an endothelial cell, which lines the inside of blood vessels and which has been labelled with different fluorescence dyes. The fluorescent dyes are chosen to highlight individual cell components by specific colours. On this image, the nucleus is blue, the membranes green and the cytosol red. The cell has a diameter of around 50 micrometres (0.05 millimetres). Taken by Martin Reifarth/Jena Centre for Soft Matter Catalyst layer The scanning electron microscopy image shows a catalyst layer made of activated nickel ac­ etate. A layer of nickel acetate is heated up using microwave radiation until the thick »carpets« made of small metal nanoparticles are formed. They can be used to synthesize carbon nanotubes, for example. The picture section has a width of around 50 micrometres (0.05 millimetres). Taken by Almut Schwenke/Jena Centre for Soft Matter 7 9 11 8 10 12 25 µm 2 µm 100 nm F E AT U R E